Previous studies have shown that oral administration of NMDAR modulators NYX-2925 reduces pain in several animal models of neuropathic pain and this appears to be through Mpfc, but no spine, mediated mechanism. While much is known about the impact of neuropathic pain in NMDAR-mediated signaling in the spinal cord, limited studies have focused on the brain. In the current study, we estimate indicates changes associated with NMDAR-mediated plasticity in Mpfc and impact of NYX-2925 administration on normalization of the signal changes.
We found decreased levels of Src Mpfc activated in animals with chronic constriction injury (CCI) of the sciatic nerve.
While Src-mediated activation of NMDARs also decreased in animals CCI, major phosphorylation sites CAMKII NMDAR not affected. This is contrary to what has been found in the spinal cord, where both Src and activation CAMKII increased. NYX oral-2925 restored the level of Src and Src phosphorylation sites enabled on GluN2A and GluN2B in Mpfc, with no effect on the level of CAMKII activated. Analgesic effect of NYX-2925 appears dependent on activation of Src in this recovery Mpfc, as co-organizer of Src inhibitor prevents activation of NYX-2925 analgesic effect.
The NMDAR modulator NYX-2925 alleviates neuropathic pain via a Src-dependent mechanism in the mPFC.
Overall, these data suggest that NMDAR-mediated signaling plays a key role in neuropathic pain, although in a different direction in the spinal cord vs Mpfc. Additionally, the analgesic effect of NYX-2925 appears to involve the recovery of NMDAR-mediated signaling in Mpfc.
TUBAL3 antibody
10R-6175
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6176
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6177
Fitzgerald
100 ul
EUR 726
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6178
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6179
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6180
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-6181
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-7226
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 antibody
10R-7227
Fitzgerald
100 ul
EUR 691
Description: Mouse monoclonal TUBAL3 antibody
TUBAL3 Antibody
1-CSB-PA025315LA01HU
Cusabio
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
TUBAL3 siRNA
20-abx938556
Abbexa
TUBAL3 siRNA
20-abx938557
Abbexa
TUBAL3 cloning plasmid
CSB-CL025315HU-10ug
Cusabio
10ug
EUR 233
Description: A cloning plasmid for the TUBAL3 gene.
TUBAL3 Antibody (HRP)
20-abx305829
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
TUBAL3 Antibody (FITC)
20-abx305830
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
TUBAL3 Antibody (Biotin)
20-abx305831
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
TUBAL3 Polyclonal Antibody
A63728
EpiGentek
100 µg
EUR 570.55
Description: kits suitable for this type of research
TUBAL3 Antibody, HRP conjugated
1-CSB-PA025315LB01HU
Cusabio
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
TUBAL3 Antibody, FITC conjugated
1-CSB-PA025315LC01HU
Cusabio
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
TUBAL3 Antibody, Biotin conjugated
1-CSB-PA025315LD01HU
Cusabio
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
TUBAL3 Recombinant Protein (Rat)
RP235271
ABM
100 ug
Ask for price
TUBAL3 Recombinant Protein (Human)
RP044566
ABM
100 ug
Ask for price
TUBAL3 Recombinant Protein (Mouse)
RP182231
ABM
100 ug
Ask for price
Mouse TUBAL3 shRNA Plasmid
20-abx982350
Abbexa
Human TUBAL3 shRNA Plasmid
20-abx962618
Abbexa
Tubal3 ORF Vector (Rat) (pORF)
ORF078425
ABM
1.0 ug DNA
EUR 506
TUBAL3 ORF Vector (Human) (pORF)
ORF014856
ABM
1.0 ug DNA
EUR 354
Tubal3 ORF Vector (Mouse) (pORF)
ORF060745
ABM
1.0 ug DNA
EUR 506
TUBAL3 Polyclonal Antibody, HRP Conjugated
A63729
EpiGentek
100 µg
EUR 570.55
Description: fast delivery possible
TUBAL3 Polyclonal Antibody, FITC Conjugated
A63730
EpiGentek
100 µg
EUR 570.55
Description: reagents widely cited
TUBAL3 Polyclonal Antibody, Biotin Conjugated
A63731
EpiGentek
100 µg
EUR 570.55
Description: Ask the seller for details
Tubal3 sgRNA CRISPR Lentivector set (Rat)
K6738401
ABM
3 x 1.0 ug
EUR 339
Tubal3 sgRNA CRISPR Lentivector set (Mouse)
K3293201
ABM
3 x 1.0 ug
EUR 339
TUBAL3 sgRNA CRISPR Lentivector set (Human)
K2558001
ABM
3 x 1.0 ug
EUR 339
Tubal3 3'UTR Luciferase Stable Cell Line
TU121356
ABM
1.0 ml
Ask for price
TUBAL3 3'UTR GFP Stable Cell Line
TU077513
ABM
1.0 ml
EUR 1394
Tubal3 3'UTR GFP Stable Cell Line
TU171356
ABM
1.0 ml
Ask for price
Tubal3 3'UTR Luciferase Stable Cell Line
TU222653
ABM
1.0 ml
Ask for price
TUBAL3 3'UTR Luciferase Stable Cell Line
TU027513
ABM
1.0 ml
EUR 1394
Tubal3 3'UTR GFP Stable Cell Line
TU272653
ABM
1.0 ml
Ask for price
TUBAL3 Protein Vector (Mouse) (pPB-C-His)
PV242978
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Mouse) (pPB-N-His)
PV242979
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Mouse) (pPM-C-HA)
PV242980
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Mouse) (pPM-C-His)
PV242981
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Rat) (pPB-C-His)
PV313698
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Rat) (pPB-N-His)
PV313699
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Rat) (pPM-C-HA)
PV313700
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Rat) (pPM-C-His)
PV313701
ABM
500 ng
EUR 603
TUBAL3 Protein Vector (Human) (pPB-C-His)
PV059421
ABM
500 ng
EUR 481
TUBAL3 Protein Vector (Human) (pPB-N-His)
PV059422
ABM
500 ng
EUR 481
TUBAL3 Protein Vector (Human) (pPM-C-HA)
PV059423
ABM
500 ng
EUR 481
TUBAL3 Protein Vector (Human) (pPM-C-His)
PV059424
ABM
500 ng
EUR 481
Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6738402
ABM
1.0 ug DNA
EUR 154
Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6738403
ABM
1.0 ug DNA
EUR 154
Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6738404
ABM
1.0 ug DNA
EUR 154
Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3293202
ABM
1.0 ug DNA
EUR 154
Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3293203
ABM
1.0 ug DNA
EUR 154
Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3293204
ABM
1.0 ug DNA
EUR 154
TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 1)
K2558002
ABM
1.0 ug DNA
EUR 154
TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 2)
K2558003
ABM
1.0 ug DNA
EUR 154
TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 3)
K2558004
ABM
1.0 ug DNA
EUR 154
Tubulin Alpha Chain-Like 3 (TUBAL3) Antibody
20-abx301309
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Tubulin Alpha Chain-Like 3 (TUBAL3) Antibody
abx145785-100ug
Abbexa
100 ug
EUR 391
TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV624343
ABM
1.0 ug DNA
EUR 682
TUBAL3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV624347
ABM
1.0 ug DNA
EUR 682
TUBAL3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV624348
ABM
1.0 ug DNA
EUR 682
Human Tubulin alpha chain- like 3, TUBAL3 ELISA KIT
ELI-52873h
Lifescience Market
96 Tests
EUR 824
Mouse Tubulin alpha chain- like 3, Tubal3 ELISA KIT
ELI-46468m
Lifescience Market
96 Tests
EUR 865
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6738405
ABM
3 x 1.0 ug
EUR 376
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K3293205
ABM
3 x 1.0 ug
EUR 376
TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K2558005
ABM
3 x 1.0 ug
EUR 376
TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV624344
ABM
1.0 ug DNA
EUR 682
TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV624345
ABM
1.0 ug DNA
EUR 740
TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV624346
ABM
1.0 ug DNA
EUR 740
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6738406
ABM
1.0 ug DNA
EUR 167
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6738407
ABM
1.0 ug DNA
EUR 167
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6738408
ABM
1.0 ug DNA
EUR 167
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K3293206
ABM
1.0 ug DNA
EUR 167
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K3293207
ABM
1.0 ug DNA
EUR 167
Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K3293208
ABM
1.0 ug DNA
EUR 167
TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K2558006
ABM
1.0 ug DNA
EUR 167
TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K2558007
ABM
1.0 ug DNA
EUR 167
TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K2558008
ABM
1.0 ug DNA
EUR 167