The NMDAR modulator NYX-2925 alleviates neuropathic pain via a Src-dependent mechanism in the mPFC.

Previous studies have shown that oral administration of NMDAR modulators NYX-2925 reduces pain in several animal models of neuropathic pain and this appears to be through Mpfc, but no spine, mediated mechanism. While much is known about the impact of neuropathic pain in NMDAR-mediated signaling in the spinal cord, limited studies have focused on the brain. In the current study, we estimate indicates changes associated with NMDAR-mediated plasticity in Mpfc and impact of NYX-2925 administration on normalization of the signal changes.

We found decreased levels of Src Mpfc activated in animals with chronic constriction injury (CCI) of the sciatic nerve.

While Src-mediated activation of NMDARs also decreased in animals CCI, major phosphorylation sites CAMKII NMDAR not affected. This is contrary to what has been found in the spinal cord, where both Src and activation CAMKII increased. NYX oral-2925 restored the level of Src and Src phosphorylation sites enabled on GluN2A and GluN2B in Mpfc, with no effect on the level of CAMKII activated. Analgesic effect of NYX-2925 appears dependent on activation of Src in this recovery Mpfc, as co-organizer of Src inhibitor prevents activation of NYX-2925 analgesic effect.

Overall, these data suggest that NMDAR-mediated signaling plays a key role in neuropathic pain, although in a different direction in the spinal cord vs Mpfc. Additionally, the analgesic effect of NYX-2925 appears to involve the recovery of NMDAR-mediated signaling in Mpfc.

TUBAL3 antibody
10R-6175	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6176	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6177	Fitzgerald	100 ul	EUR 726
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6178	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6179	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6180	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-6181	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-7226	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

TUBAL3 antibody
10R-7227	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal TUBAL3 antibody

anti-TUBAL3
YF-PA20927	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to TUBAL3

anti-TUBAL3
YF-PA20928	Abfrontier	100 ul	EUR 403
Description: Rabbit polyclonal to TUBAL3

anti-TUBAL3
YF-PA20929	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to TUBAL3

TUBAL3 cloning plasmid
CSB-CL025315HU-10ug	Cusabio	10ug	EUR 233
Description: A cloning plasmid for the TUBAL3 gene.

TUBAL3 Antibody (HRP)
20-abx305829	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

TUBAL3 Antibody (FITC)
20-abx305830	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

TUBAL3 Antibody (Biotin)
20-abx305831	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

TUBAL3 Polyclonal Antibody
A63728	EpiGentek	100 µg	EUR 570.55
Description: kits suitable for this type of research

TUBAL3 Antibody, HRP conjugated
1-CSB-PA025315LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

TUBAL3 Antibody, FITC conjugated
1-CSB-PA025315LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

TUBAL3 Antibody, Biotin conjugated
1-CSB-PA025315LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against TUBAL3. Recognizes TUBAL3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Human TUBAL3 shRNA Plasmid
20-abx962618	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Mouse TUBAL3 shRNA Plasmid
20-abx982350	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

TUBAL3 Recombinant Protein (Human)
RP044566	ABM	100 ug	Ask for price

TUBAL3 Recombinant Protein (Mouse)
RP182231	ABM	100 ug	Ask for price

TUBAL3 Recombinant Protein (Rat)
RP235271	ABM	100 ug	Ask for price

TUBAL3 Polyclonal Antibody, HRP Conjugated
A63729	EpiGentek	100 µg	EUR 570.55
Description: fast delivery possible

TUBAL3 Polyclonal Antibody, FITC Conjugated
A63730	EpiGentek	100 µg	EUR 570.55
Description: reagents widely cited

TUBAL3 Polyclonal Antibody, Biotin Conjugated
A63731	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

TUBAL3 ORF Vector (Human) (pORF)
ORF014856	ABM	1.0 ug DNA	EUR 354

Tubal3 ORF Vector (Mouse) (pORF)
ORF060745	ABM	1.0 ug DNA	EUR 506

Tubal3 ORF Vector (Rat) (pORF)
ORF078425	ABM	1.0 ug DNA	EUR 506

Tubal3 sgRNA CRISPR Lentivector set (Mouse)
K3293201	ABM	3 x 1.0 ug	EUR 339

Tubal3 sgRNA CRISPR Lentivector set (Rat)
K6738401	ABM	3 x 1.0 ug	EUR 339

TUBAL3 sgRNA CRISPR Lentivector set (Human)
K2558001	ABM	3 x 1.0 ug	EUR 339

Tubulin Alpha Chain-Like 3 (TUBAL3) Antibody
abx145785-100ug	Abbexa	100 ug	EUR 391

Tubulin Alpha Chain-Like 3 (TUBAL3) Antibody
20-abx301309	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3293202	ABM	1.0 ug DNA	EUR 154

Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3293203	ABM	1.0 ug DNA	EUR 154

Tubal3 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3293204	ABM	1.0 ug DNA	EUR 154

Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6738402	ABM	1.0 ug DNA	EUR 154

Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6738403	ABM	1.0 ug DNA	EUR 154

Tubal3 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6738404	ABM	1.0 ug DNA	EUR 154

TUBAL3 3'UTR GFP Stable Cell Line
TU077513	ABM	1.0 ml	EUR 1394

TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 1)
K2558002	ABM	1.0 ug DNA	EUR 154

TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 2)
K2558003	ABM	1.0 ug DNA	EUR 154

TUBAL3 sgRNA CRISPR Lentivector (Human) (Target 3)
K2558004	ABM	1.0 ug DNA	EUR 154

TUBAL3 Protein Vector (Rat) (pPB-C-His)
PV313698	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Rat) (pPB-N-His)
PV313699	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Rat) (pPM-C-HA)
PV313700	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Rat) (pPM-C-His)
PV313701	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Human) (pPB-C-His)
PV059421	ABM	500 ng	EUR 481

TUBAL3 Protein Vector (Human) (pPB-N-His)
PV059422	ABM	500 ng	EUR 481

TUBAL3 Protein Vector (Human) (pPM-C-HA)
PV059423	ABM	500 ng	EUR 481

TUBAL3 Protein Vector (Human) (pPM-C-His)
PV059424	ABM	500 ng	EUR 481

TUBAL3 Protein Vector (Mouse) (pPB-C-His)
PV242978	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Mouse) (pPB-N-His)
PV242979	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Mouse) (pPM-C-HA)
PV242980	ABM	500 ng	EUR 603

TUBAL3 Protein Vector (Mouse) (pPM-C-His)
PV242981	ABM	500 ng	EUR 603

Tubal3 3'UTR Luciferase Stable Cell Line
TU222653	ABM	1.0 ml	Ask for price

Tubal3 3'UTR GFP Stable Cell Line
TU171356	ABM	1.0 ml	Ask for price

Tubal3 3'UTR GFP Stable Cell Line
TU272653	ABM	1.0 ml	Ask for price

TUBAL3 3'UTR Luciferase Stable Cell Line
TU027513	ABM	1.0 ml	EUR 1394

Tubal3 3'UTR Luciferase Stable Cell Line
TU121356	ABM	1.0 ml	Ask for price

TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV624343	ABM	1.0 ug DNA	EUR 682

TUBAL3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV624347	ABM	1.0 ug DNA	EUR 682

TUBAL3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV624348	ABM	1.0 ug DNA	EUR 682

Human Tubulin alpha chain- like 3, TUBAL3 ELISA KIT
ELI-52873h	Lifescience Market	96 Tests	EUR 824

Mouse Tubulin alpha chain- like 3, Tubal3 ELISA KIT
ELI-46468m	Lifescience Market	96 Tests	EUR 865

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K3293205	ABM	3 x 1.0 ug	EUR 376

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6738405	ABM	3 x 1.0 ug	EUR 376

TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K2558005	ABM	3 x 1.0 ug	EUR 376

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K3293206	ABM	1.0 ug DNA	EUR 167

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K3293207	ABM	1.0 ug DNA	EUR 167

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K3293208	ABM	1.0 ug DNA	EUR 167

TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV624344	ABM	1.0 ug DNA	EUR 682

TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV624345	ABM	1.0 ug DNA	EUR 740

TUBAL3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV624346	ABM	1.0 ug DNA	EUR 740

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6738406	ABM	1.0 ug DNA	EUR 167

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6738407	ABM	1.0 ug DNA	EUR 167

Tubal3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6738408	ABM	1.0 ug DNA	EUR 167

TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K2558006	ABM	1.0 ug DNA	EUR 167

TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K2558007	ABM	1.0 ug DNA	EUR 167

TUBAL3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K2558008	ABM	1.0 ug DNA	EUR 167